D-erythrose-[1-d]

D-erythrose-[1-d] is a labeled form of D-erythrose used extensively in biochemical and metabolic studies. Here are some key applications of D-erythrose-[1-d]:

Metabolic Tracing: D-erythrose-[1-d] is utilized in metabolic tracing experiments to study carbohydrate metabolism and the pentose phosphate pathway. By incorporating this labeled sugar into metabolic pathways, researchers can trace its conversion and breakdown within cells. This information is vital in understanding metabolic fluxes and identifying pathway bottlenecks.

Glycosylation Studies: This labeled sugar is used to examine glycosylation processes, where sugars are added to proteins and lipids. Researchers incorporate D-erythrose-[1-d] into cellular systems to trace glycan modification and distribution. This helps in understanding the roles of glycosylation in diseases and developing novel therapeutic strategies.

Enzyme Kinetics: D-erythrose-[1-d] can be employed to study enzyme kinetics and substrate specificity of enzymes involved in carbohydrate metabolism. By using the labeled substrate, researchers can measure the rate of enzymatic reactions and identify active sites. These studies contribute to the development of enzyme inhibitors and activators that can regulate metabolic disorders.

Isotope Dilution Analysis: In analytical chemistry, D-erythrose-[1-d] is applied in isotope dilution analysis for precise quantification of sugars in complex mixtures. By adding a known quantity of this labeled sugar as an internal standard, accurate measurements of naturally occurring erythrose can be achieved. This technique is fundamental in quality control and biochemical assays.