D-Arabinose-[1,5-13C2]

D-Arabinose-[1,5-13C2], a specialized labeled sugar molecule, finds diverse applications in biochemical and metabolic research. Here are the key applications of D-Arabinose-[1,5-13C2]:

Metabolic Flux Analysis: Utilized in metabolic flux analysis, D-Arabinose-[1,5-13C2] serves as a tool for scrutinizing the pathways and velocities of metabolic reactions within cells. By monitoring the integration of the 13C label into metabolic intermediates, researchers can elucidate the carbon flow across various metabolic pathways. This approach unveils insights into cellular metabolism, revealing potential targets for metabolic engineering endeavors.

NMR Spectroscopy: Embracing this labeled sugar in nuclear magnetic resonance (NMR) spectroscopy enables in-depth exploration of carbohydrate structures and dynamics. The 13C labeling amplifies NMR signals, facilitating meticulous examinations of molecular conformations and interactions. Such insights are paramount for unraveling the intricacies of sugar chemistry and its pivotal role in biological systems.

Glycosylation Studies: In the realm of glycosylation studies, D-Arabinose-[1,5-13C2] emerges as a valuable asset for investigating the processes where sugars bind to proteins and lipids. By employing labeled sugars, scientists can track the integration and alteration patterns of glycosyl groups, shedding light on the biosynthesis and functional significance of glycoconjugates in cellular processes.

Isotopic Labeling in Biochemistry: Through the integration of D-Arabinose-[1,5-13C2] into biochemical experiments, researchers delve into the mechanisms of enzymes and interactions with substrates. Isotopic labeling offers precise monitoring of molecular transformations and enzyme kinetics, playing a pivotal role in unraveling the catalytic activity and regulation of carbohydrate-modifying enzymes.