D-Mannose-[3,4-13C2]

D-Mannose-[3,4-13C2] is a stable isotope-labeled sugar used primarily in research to track and analyze metabolic pathways. Here are some key applications of D-Mannose-[3,4-13C2]:

Metabolic Flux Analysis: D-Mannose-[3,4-13C2] can be utilized in metabolic flux analysis to trace the metabolic pathways in various organisms, including humans. By following the isotopic enrichment in metabolic intermediates, researchers can quantify the fluxes through central carbon metabolism. This information is crucial for understanding cellular metabolism and optimizing metabolic networks for biotechnological applications.

Glycosylation Studies: This isotope-labeled mannose is used in glycosylation studies to investigate the biosynthesis and regulation of glycoproteins and glycolipids. By incorporating D-Mannose-[3,4-13C2] into glycan structures, scientists can track and analyze the glycosylation patterns and site-specific modifications. This is essential for understanding the role of glycosylation in health, disease, and therapeutic protein production.

Diagnostic Research: In diagnostic research, D-Mannose-[3,4-13C2] serves as a tracer to study disorders related to carbohydrate metabolism, such as congenital disorders of glycosylation (CDG). Patients are administered the labeled sugar, and the metabolic fate is monitored through biofluid sampling and mass spectrometry. This helps in diagnosing and understanding the pathophysiology of metabolic diseases.

Nutritional Research: D-Mannose-[3,4-13C2] is used to investigate the absorption and metabolic fate of dietary sugars in nutritional studies. By labeling dietary mannose, researchers can study its impact on gut microbiota absorption rates and metabolic conversions in the body. This application aids in comprehensively understanding the role of mannose in human nutrition and metabolic health.