D-erythrose-[1-13C]

D-erythrose-[1-13C], a stable isotope-labeled compound, finds diverse applications in scientific research. Here are key applications presented with a high degree of perplexity and burstiness:

Metabolic Tracing Studies: Playing a pivotal role in metabolic tracing experiments, D-erythrose-[1-13C] facilitates the tracking of carbon flow through intricate metabolic pathways. By integrating this labeled compound into biological systems, researchers can meticulously monitor and quantify carbon assimilation, offering profound insights into metabolic distributions and pathway activities. This comprehension is crucial for unraveling the complexities of cellular metabolism and discerning the repercussions of diverse treatments or genetic modifications.

NMR Spectroscopy: With its application in nuclear magnetic resonance (NMR) spectroscopy, D-erythrose-[1-13C] serves as a critical tool in studying the structures and dynamics of carbohydrates. The 13C enrichment enhances NMR signal sensitivity and resolution, enabling in-depth exploration of molecular interactions and conformational changes. Scientists heavily rely on this technique to decipher the intricacies of carbohydrate biosynthesis and degradation processes, pushing the boundaries of structural biology.

Stable Isotope-Labeled Standards: Acting as a gold standard in mass spectrometry, D-erythrose-[1-13C] functions as a reference compound for the quantitative analysis of metabolic landscapes. By juxtaposing the labeled compound against its natural counterpart, researchers can precisely quantify fluctuations in metabolite levels across varying experimental conditions. This methodology proves invaluable in biomarker discovery and metabolic profiling, offering a profound understanding of physiological changes at a molecular level.

Biochemical Research: In the realm of biochemical investigations, D-erythrose-[1-13C] emerges as a valuable asset for studying enzymatic reactions involving sugar molecules. The isotope label plays a crucial role in tracing enzyme-driven transformations and pinpointing reaction intermediates, shedding light on enzyme mechanisms. These insights not only deepen our comprehension of enzymatic processes but also pave the way for designing inhibitors or modulators with therapeutic implications, highlighting the compound's significance in biochemical research.