D-Fructose-[2,5-13C2]

D-Fructose-[2,5-13C2], a specialized form of fructose with isotopic labeling, finds diverse applications in scientific research. Here are the key applications of D-Fructose-[2,5-13C2]:

Metabolic Flux Analysis: In the realm of metabolic flux analysis, D-Fructose-[2,5-13C2] shines as a tool for scrutinizing carbon flow within metabolic pathways. By monitoring the incorporation of labeled carbon atoms, researchers unveil the workings of specific metabolic routes. This technique plays a pivotal role in unraveling the dynamic intricacies and regulatory mechanisms governing diverse biological systems.

NMR Spectroscopy: Delving into the realm of nuclear magnetic resonance (NMR) spectroscopy, D-Fructose-[2,5-13C2] emerges as a key player in probing molecular structures and dynamics. The 13C label offers distinct signals that aid in deciphering the configuration and conformation of fructose in solution. This application stands as a cornerstone in structural biology and the exploration of carbohydrate chemistry, offering a window into the molecular ballet within biological systems.

Stable Isotope Tracing: Stepping into the domain of biochemical research, D-Fructose-[2,5-13C2] takes center stage in stable isotope tracing experiments, shedding light on nutrient assimilation and metabolic conversions. Tracing the labeled fructose through various metabolic pathways unveils insights into carbohydrate metabolism and energy generation. This methodology proves invaluable in investigations centered on metabolic disorders and the nuances of nutritional science, painting a detailed portrait of metabolic intricacies.

Gut Microbiota Studies: Venturing into the intriguing realm of gut microbiota studies, D-Fructose-[2,5-13C2] offers a lens into the metabolism of fructose by the microbial inhabitants of our intestines. By administering labeled fructose to experimental subjects, researchers embark on a journey to decipher how gut bacteria harness and transform fructose. This application stands as a cornerstone in unraveling the intricate dance between diet, microbiota, and host metabolism, shedding light on the hidden interactions that shape our internal ecosystem.