D-xylulose-[UL-13C5]

D-xylulose-[UL-13C5], a carbon-13 isotopic variant of D-xylulose, finds extensive utility in metabolic research. Here are the key applications:

Metabolic Flux Analysis: This labeled compound serves as a versatile tool for tracing metabolic routes and quantifying carbon flow across diverse metabolic grids. By infusing this labeled sugar into cellular matrices, researchers can meticulously track its metabolization into other compounds using cutting-edge mass spectrometry or NMR spectroscopy techniques. This detailed scrutiny unveils intricate cellular metabolic landscapes, revolutionizing the exploration of metabolic anomalies and bolstering bioengineering endeavors.

Nutritional Studies: Embark on a journey through carbohydrate metabolism and the metabolic repercussions of varying dietary regimens using D-xylulose-[UL-13C5]. Administering this labeled sugar to animal models or human volunteers elucidates the processing and utilization of dietary sugars in the body. This empirical data sheds light on the nutritional quality and metabolic ramifications of specific dietary constituents, reshaping our comprehension of metabolic dynamics.

Enzyme Activity Assays: Witness the power of D-xylulose-[UL-13C5] in decoding enzyme functionality within pentose phosphate and allied pathways. By presenting a labeled substrate, researchers can monitor enzymatic conversions with unparalleled precision, quantifying enzyme kinetics with finesse. This precision aids in unveiling enzyme roles and pinpointing potential metabolic bottlenecks, propelling enzyme research into uncharted territories.

Biotechnology and Bioengineering: Step into the realm of industrial biotechnology where D-xylulose-[UL-13C5] serves as a linchpin for refining microbial manufacturing processes and augmenting biochemical synthesis. Tracing the journey of labeled carbon atoms empowers scientists to optimize metabolic routes, boosting the output of desired biomolecules. This strategy catalyzes the evolution of sustainable bio-manufacturing protocols and advances in strain engineering, shaping the future of bio-based industries.