Guanosine Monophosphate-[13C5] Monosodium Salt

Guanosine Monophosphate-[13C5] Monosodium Salt is a stable isotope-labeled compound widely used in bioscience research and various analytical applications. Here are some key applications of Guanosine Monophosphate-[13C5] Monosodium Salt:

Metabolic Tracing Studies: This labeled compound is frequently used in metabolic studies to trace biochemical pathways. By incorporating it into cells or organisms, researchers can monitor the metabolic fate and transformations of GMP in vivo and in vitro. These studies are essential for understanding nucleotide metabolism and its implications in health and disease.

NMR Spectroscopy: The [13C5] labeling of Guanosine Monophosphate provides a distinct signature for nuclear magnetic resonance (NMR) spectroscopy, enabling detailed structural and dynamic analyses. It allows researchers to investigate interactions and conformational changes involving GMP at the atomic level. This application is particularly significant in structural biology and the study of complex biomolecules.

Quantitative Mass Spectrometry: Guanosine Monophosphate-[13C5] serves as an important internal standard in mass spectrometry-based quantification of nucleotides. Its stable isotope label facilitates accurate measurement of GMP concentrations within various biological samples. This precision is crucial in pharmacokinetic studies and for elucidating cellular nucleotide pools.

Biochemical Reaction Mechanism Studies: The incorporation of Guanosine Monophosphate-[13C5] in enzyme studies aids in elucidating reaction mechanisms and enzyme kinetics. Researchers can track the fate of carbon within the guanosine molecule during enzymatic conversions. This insight helps refine our understanding of enzymatic processes and the roles of nucleotides in cellular biochemistry.