Methyl β-D-glucopyranoside-[1-13C]

Methyl β-D-glucopyranoside-[1-13C], a stable isotopically labeled compound, finds diverse biotechnological and analytical applications. Here are the key applications of Methyl β-D-glucopyranoside-[1-13C]:

Metabolic Flux Analysis: Utilizing Methyl β-D-glucopyranoside-[1-13C] for metabolic flux analysis allows researchers to trace carbon pathways within cellular systems. By integrating this labeled compound into metabolic investigations, scientists can monitor the distribution and transformation of 13C-labeled carbons. This methodology aids in comprehending and mapping intricate metabolic networks, providing profound insights into cellular metabolism and bioenergetics.

NMR Spectroscopy: Within nuclear magnetic resonance (NMR) spectroscopy, Methyl β-D-glucopyranoside-[1-13C] acts as a pivotal probe for exploring carbohydrate structures and dynamics. The 13C label heightens NMR signal sensitivity, enabling comprehensive analysis of glycosidic linkages and conformational changes. This application holds paramount importance in structural biology and the advancement of carbohydrate-based therapeutics, unlocking new avenues for research and development.

Enzyme Kinetics: Researchers leverage Methyl β-D-glucopyranoside-[1-13C] to delve into the kinetic properties of glycosidases and other carbohydrate-acting enzymes. By monitoring the reactions of the labeled substrate, scientists can determine enzyme activity, binding affinity, and catalytic mechanisms. These investigations play a pivotal role in enzyme engineering and biocatalysis research, shedding light on the complex interactions underlying enzymatic processes.

Stable Isotope Labeling: In the realm of stable isotope labeling applications, Methyl β-D-glucopyranoside-[1-13C] serves as a benchmark reference or a labeled constituent in metabolic engineering experiments. This compound aids in quantifying metabolic fluxes and evaluating the rates of carbon atom integration into diverse biomolecules. Such data is crucial for optimizing metabolic pathways and enhancing yields in industrial biotechnology, driving innovation and progress in the field.