Ribitol-[13C5], a crucial stable isotope-labeled compound, finds extensive use in metabolic research and tracer studies. Here are the key applications of Ribitol-[13C5]:
Metabolomics: In the realm of mass spectrometry-based metabolomics, Ribitol-[13C5] takes center stage as an internal standard ensuring the precise quantitation of metabolites. By incorporating this isotope-labeled compound, researchers can mitigate variability in sample preparation and analysis, enhancing the reliability of metabolic data. This approach facilitates the identification and quantification of metabolic changes, unveiling intricate metabolic pathways.
Flux Analysis: Serving as a dynamic tracer in metabolic flux analysis, Ribitol-[13C5] enables the study of metabolic pathway dynamics. By tracing the incorporation of labeled carbon atoms into diverse metabolic intermediates, scientists can meticulously chart the flow of metabolites through different pathways. This comprehensive analysis provides deep insights into cellular metabolism, shedding light on how cells adeptly respond to varying physiological conditions.
Enzyme Mechanism Studies: Delving into the catalytic processes of ribitol-utilizing enzymes, Ribitol-[13C5] plays a pivotal role in enzyme kinetics and mechanism studies. The stable isotope labeling ensures precise monitoring of substrate conversion and product formation, offering a clear window into enzyme function, reaction mechanisms, and regulatory properties. This analytical approach contributes significantly to our understanding of enzymatic processes, unraveling the intricacies of biochemical reactions.
![Ribitol-[13C5]](/isotope.v1/images/structure-default.jpg)
