L-Aspartic acid-[1,4-13C2,15N]

L-Aspartic acid-[1,4-13C2,15N], a stable isotope-labeled amino acid with diverse scientific applications, is at the forefront of cutting-edge research. Here are the key applications of L-Aspartic acid-[1,4-13C2,15N]:

Metabolic Flux Analysis: In the realm of metabolic flux analysis, L-Aspartic acid-[1,4-13C2,15N] plays a pivotal role in tracing intricate metabolic pathways within cells. By integrating this labeled amino acid, researchers can meticulously track the movement of carbon and nitrogen through complex metabolic networks. This profound insight into cellular metabolism allows for the identification of bottlenecks in metabolic engineering studies, paving the way for optimized pathways and enhanced bioengineering solutions.

Protein Structure and Dynamics: Delving into the realm of protein structure and dynamics, the incorporation of L-Aspartic acid-[1,4-13C2,15N] into proteins unlocks a realm of possibilities for scientists. This isotopic labeling strategy facilitates detailed studies of protein folding, structure, and dynamics using cutting-edge NMR spectroscopy techniques. The distinct signals generated by isotopic labeling provide valuable insights into the precise location and environment of the labeled amino acid within the protein, offering critical information for unraveling protein mechanisms and interactions.

Enzyme Mechanism Studies: At the heart of enzyme mechanism studies, isotope-labeled L-Aspartic acid serves as a powerful tool for investigating enzymatic reactions and mechanisms in great detail. By replacing conventional aspartic acid with the labeled variant, researchers can meticulously track isotope shifts in substrates and products, unveiling intricate insights into enzyme catalytic cycles. This deep understanding of enzyme function is not only essential for drug design, but also plays a crucial role in advancing our knowledge of enzyme mechanisms, opening doors to innovative therapeutic interventions.

Biological Pathway Elucidation: Within the realm of systems biology, L-Aspartic acid-[1,4-13C2,15N] emerges as a key player in mapping out and quantifying fluxes through diverse biochemical pathways. This powerful tracer aids in constructing detailed models of cellular metabolism, offering crucial insights into disease states and laying the foundation for the development of targeted therapeutic strategies. By unraveling the intricate web of biological pathways, researchers can guide interventions aimed at manipulating pathway activity to achieve desired outcomes, shaping the future of precision medicine and personalized healthcare.