D-ribose-[1-13C,1-d]

D-ribose-[1-13C,1-d], a specialized form of D-ribose extensively utilized in metabolic and biochemical research, offers a myriad of applications. Here are the key applications:

NMR Spectroscopy: Delving into nuclear magnetic resonance (NMR) spectroscopy, D-ribose-[1-13C,1-d] emerges as a crucial tool for probing carbohydrate metabolism and structural biology. The strategic 13C-label enables researchers to meticulously track the carbon atom along metabolic pathways, unveiling deep insights into ribose's cellular utilization. This knowledge is pivotal for deciphering the dynamics of nucleotide biosynthesis and the intricate dance of energy metabolism within living cells.

Metabolic Flux Analysis: Enter the realm of metabolic engineering, where D-ribose-[1-13C,1-d] takes center stage in facilitating metabolic flux analysis. By incorporating this tagged compound into cellular systems, scientists can quantitatively measure the carbon flow across diverse metabolic routes. This data proves instrumental in optimizing metabolic pathways to boost the production yields of sought-after products in biotechnology realms like biofuels and pharmaceuticals, ushering in a new era of efficiency and productivity.

Stable Isotope Labeling: Witness the transformative power of D-ribose-[1-13C,1-d] as a stable isotope-labeled compound in the arena of mass spectrometry (MS) studies. This application shines in dissecting the incorporation of ribose into nucleotides and nucleic acids, shedding light on the kinetics of nucleotide turnover and the intricate orchestration of DNA and RNA synthesis. Such studies hold the key to unraveling the mysteries of molecular dynamics within cellular machinery, paving the way for groundbreaking discoveries and advancements.

Biochemical Pathway Elucidation: Embark on a journey of biochemical exploration as researchers harness D-ribose-[1-13C,1-d] to map and illuminate intricate pathways involving ribose. By integrating this labeled ribose into cellular milieus, scientists meticulously unravel the enzymatic choreography that transforms ribose into diverse metabolites. This illuminates pivotal regulatory points within the pentose phosphate pathway and nucleotide metabolism, underpinning a profound comprehension of cell physiology and metabolic intricacies at play.