D-Galactose-[1-18O]

D-Galactose-[1-18O] is an isotopically labeled variation of D-Galactose that finds primary utility in metabolic and biochemical research. Here are its key applications:

Metabolic Pathway Tracing: Delving into metabolic intricacies, D-Galactose-[1-18O] emerges as a pivotal tool in tracing galactose flux through intricate pathways. By infusing this labeled sugar into biological systems, researchers can meticulously monitor its journey and transformation into diverse metabolites. This insight plays a critical role in unraveling the mysteries of carbohydrate metabolism and associated disorders.

Enzyme Mechanism Studies: At the forefront of enzyme exploration, D-Galactose-[1-18O] stands as a beacon for uncovering the inner workings of galactose-metabolizing enzymes. Through meticulous scrutiny of the labeled oxygen atom's integration and turnover, researchers glean valuable understandings of enzyme activity and catalytic mechanisms. These investigations play a pivotal role in demystifying enzyme functionalities and sculpting inhibitors or activators for therapeutic pursuits.

Glycoprotein Analysis: Within the realm of glycoprotein scrutiny, D-Galactose-[1-18O] serves as a cornerstone for unraveling the intricacies of glycoprotein biosynthesis and configuration. Acting as a beacon, this labeled sugar facilitates the tracking of galactose assimilation into glycoprotein oligosaccharide chains. This application serves as a lighthouse, illuminating the realm of protein glycosylation, a fundamental component of diverse biological processes and disease pathways.

Isotope Dilution Mass Spectrometry: In the realm of quantitative analysis, D-Galactose-[1-18O] emerges as a prized asset within isotope dilution mass spectrometry (IDMS). By juxtaposing the labeled compound with its unaltered counterpart, precise measurements of galactose concentrations within biological samples come to light. This technique forms a cornerstone in diagnostic realms and the monitoring of metabolic imbalances, unlocking new avenues for understanding and treating metabolic anomalies.