D-Arabinose-[3-13C]

D-Arabinose-[3-13C], a stable isotope-labeled sugar, finds diverse applications in biochemical and biophysical realms. Here are the key applications:

Metabolic Flux Analysis: Employing D-Arabinose-[3-13C] allows for tracing carbon flux within metabolic pathways. By integrating this labeled sugar into microbial or cellular cultures, researchers can meticulously track the distribution and conversion of the labeled carbon atom across various metabolic intermediates. This in-depth analysis offers valuable insights into the dynamics of metabolic networks, unveiling potential bottlenecks and regulatory nodes within pathways.

Structural Biology: Within the domain of structural biology, D-Arabinose-[3-13C] stands as a pivotal tool for labeling carbohydrates in Nuclear Magnetic Resonance (NMR) spectroscopy and other structural characterization techniques. The isotope label plays a crucial role in elucidating the three-dimensional structures of carbohydrate-protein complexes, shedding light on their interactions at an atomic level. This structural data is instrumental in drug design and deciphering the functional significance of carbohydrates within intricate biological systems.

Enzyme Mechanism Studies: Serving as a valuable asset in probing enzyme-catalyzed reactions, D-Arabinose-[3-13C] enables researchers to delve into the intricacies of enzymatic processes. By utilizing the labeled substrate, scientists can meticulously track the incorporation of the labeled carbon into reaction products through NMR or mass spectrometry. This investigative approach aids in unraveling enzyme mechanisms, pinpointing rate-limiting steps, and exploring the kinetic behaviors of enzymes involved in carbohydrate metabolism.

Agricultural Research: In the realm of agricultural studies, D-Arabinose-[3-13C] plays a pivotal role in evaluating plant metabolism and carbon distribution. Through the administration of labeled D-Arabinose to plants, researchers can elucidate the absorption, translocation, and utilization pathways of the sugar within different plant tissues. This knowledge contributes to a deeper understanding of plant growth dynamics, stress responses, and the overall efficacy of carbon utilization in agricultural crops.