D-arabinose-[3,4,5-13C3]

D-arabinose-[3,4,5-13C3], a modified form of the natural sugar D-arabinose with three carbon atoms substituted by stable carbon-13 isotopes, finds diverse applications in scientific research. Here are the key applications of D-arabinose-[3,4,5-13C3]:

Metabolic Tracing: Widely utilized in metabolic investigations, D-arabinose-[3,4,5-13C3] serves as a potent tool for tracing sugar metabolism pathways and fluxes. By introducing this labeled sugar, researchers can meticulously monitor its transformation and integration into diverse metabolic products through advanced techniques like NMR and mass spectrometry. This methodology facilitates a deeper comprehension of metabolic dynamics, shedding light on crucial regulatory nodes within biological systems.

Structural Biology: In the realm of structural biology, D-arabinose-[3,4,5-13C3] is harnessed to probe the interactions and conformations of carbohydrates within intricate biomolecules. The strategic isotope labeling permits in-depth NMR spectroscopy analyses, furnishing valuable insights into the structure and dynamics of polysaccharides, glycoproteins, and other carbohydrate-containing entities. Such investigations play a pivotal role in unraveling the functional significance of carbohydrates in intricate biological processes, enhancing our understanding of molecular intricacies.

Biochemical Pathway Elucidation: Scientists leverage D-arabinose-[3,4,5-13C3] to decipher enzyme-catalyzed reactions involving this sugar substrate. By scrutinizing the fate of the labeled carbons in reaction products, researchers glean precise details about enzymatic mechanisms and pathway intermediates. This application holds significance in characterizing enzyme functionalities and unraveling the architectural blueprint of metabolic pathways, contributing to a comprehensive comprehension of biochemical processes.

Glycomics Research: Positioned at the forefront of glycomics research, D-arabinose-[3,4,5-13C3] plays a pivotal role in studying glycan structures and functions comprehensively. Through the incorporation of labeled D-arabinose into glycans, researchers can meticulously trace the biosynthesis and remodeling of carbohydrates within cellular contexts. This sophisticated technique aids in mapping out intricate glycan pathways and exploring the functional contributions of carbohydrates in the realms of health and disease, offering profound insights into biological complexities.