D-Glucitol-[6-18O]

D-Glucitol-[6-18O], also known as D-sorbitol-[6-18O], is a specialized form of sorbitol enriched with oxygen-18 at the C-6 position. This labeled compound is utilized in diverse research and diagnostic domains. Below are the key applications of D-Glucitol-[6-18O]:

Metabolic Pathway Studies: Delving into metabolic pathways, D-Glucitol-[6-18O] offers invaluable insights into the utilization and transformation of sorbitol. By tracking the oxygen-enriched label, researchers can unravel specific biochemical reactions involving sorbitol, enhancing comprehension of carbohydrate metabolism and related disorders.

NMR Spectroscopy: Widely employed in Nuclear Magnetic Resonance (NMR) spectroscopy, D-Glucitol-[6-18O] aids in exploring molecular structures and interactions. The presence of oxygen-18 boosts NMR signal sensitivity, facilitating the analysis of conformational and structural dynamics in sorbitol-containing compounds with greater precision.

Enzyme Kinetics: Serving as a potent tool for investigating enzyme kinetics, this isotopically labeled compound is particularly beneficial for studying enzymes that metabolize sorbitol. By utilizing D-Glucitol-[6-18O], scientists can effectively monitor enzymatic reactions and determine the conversion rate of sorbitol, aiding in the characterization of enzyme mechanisms and identification of potential inhibitors.

Isotope Tracing in Plant Physiology: In the realm of plant physiology research, D-Glucitol-[6-18O] plays a pivotal role in exploring the functions of sorbitol in osmoregulation and carbon allocation. By tracing the labeled sorbitol's movement and conversion within plant systems, researchers can shed light on how plants regulate osmotic balance and allocate resources vital for enhancing crop stress resilience and productivity.