D-Allose-[1-13C]

D-Allose-[1-13C], an isotopically labeled sugar molecule, finds diverse applications in biochemical and physiological studies. Here are the key applications of D-Allose-[1-13C]:

Metabolic Flux Analysis: Delving into metabolic flux analysis, researchers utilize D-Allose-[1-13C] to trace carbohydrate metabolic pathways within live organisms. By meticulously tracking the incorporation of labeled carbon, scientists sketch out metabolic routes and quantify pathway activity in intricate detail. This knowledge plays a pivotal role in unraveling cellular metabolism nuances and pinpointing potential metabolic bottlenecks with precision.

Stable Isotope Labeling: Turning to stable isotope labeling studies, D-Allose-[1-13C] takes center stage in probing metabolic dynamics and nutrient cycling dynamics. Implementing this labeled sugar into biological systems, researchers scrutinize and quantify the transformation and utilization of carbon sources with acute accuracy. These investigations offer profound insights into the intricate regulation of metabolic processes and the interplay among diverse metabolic pathways.

NMR Spectroscopy: A valuable substrate in nuclear magnetic resonance (NMR) spectroscopy experiments, D-Allose-[1-13C] serves the purpose of elucidating carbohydrate structure and dynamics in fine detail. The labeled carbon emits a distinct signal for detection and analysis, enabling researchers to deduce the molecular conformation and interactions of the sugar in solution with meticulous precision. This sophisticated technique facilitates a profound understanding of the structural foundations of carbohydrate function, unraveling the mysteries of sugar behavior in solution.

Biosynthetic Pathway Tracing: Taking on the task of biosynthetic pathway tracing, D-Allose-[1-13C] emerges as a crucial tool in unveiling the origin and destiny of carbon atoms within complex biological molecules. By administering labeled D-Allose to organisms or cell cultures, researchers artfully trail the labeled carbon as it journeys through various biochemical reactions, shedding light on intricate biosynthetic pathways. This strategy allows researchers to pinpoint specific steps within biosynthetic pathways and decode the intricate flow of carbon through diverse biochemical networks, revolutionizing our understanding of molecular transformations.