D-fructose-[UL-13C6] 1,6-bisphosphate sodium salt

D-fructose-[UL-13C6] 1,6-bisphosphate sodium salt, a labeled biochemical compound, is extensively utilized in metabolic and physiological research. Here are the key applications:

Metabolic Pathway Elucidation: In the intricate realm of metabolic research, this compound serves as a beacon for researchers seeking to untangle the elusive glycolytic pathway across a myriad of organisms. By infusing the [UL-13C6] label into the system, experts embark on a meticulous journey to trace the meandering paths of carbon atoms through the labyrinthine metabolic network. Armed with state-of-the-art tools like NMR or mass spectrometry, this method unveils profound insights into cellular energy metabolism and the dynamic ebbs and flows of metabolic fluxes.

Enzyme Kinetics Studies: Delving into the realm of enzyme kinetics, D-fructose-[UL-13C6] 1,6-bisphosphate sodium salt emerges as a cornerstone tool for evaluating the catalytic prowess of pivotal glycolytic enzymes such as aldolase and fructose-16-bisphosphatase. By scrutinizing the intricate dance between the labeled substrate and these enzymes, researchers unveil the hidden secrets of kinetic parameters and enzyme efficiency, shedding luminous beams on enzyme functionality under a myriad of physiological scenarios.

Isotope Labeling in Mass Spectrometry: The [UL-13C6] adorned D-fructose-16-bisphosphate stands as a potent ally in the realm of mass spectrometry, enabling the precise quantification of metabolites amidst the swirling chaos of biological matrices. Acting as an internal compass or tracer, this compound simplifies the intricate web of metabolic intermediates, allowing for seamless exploration of metabolic alterations in both states of health and disease. This method ushers in a new era of non-invasive metabolic monitoring, unlocking hidden pathways to better understand the inner workings of biological systems.

Biotechnological Applications: Within the realm of bioprocessing, D-fructose-[UL-13C6] 16-bisphosphate emerges as a linchpin in the optimization of metabolic pathways for the streamlined production of biofuels and biochemicals. By harnessing the power of labeled substrates, biotechnologists embark on a quest to monitor the graceful flow of carbon through meticulously engineered pathways, fine-tuning the art of metabolic engineering for optimal efficiency. This groundbreaking advancement heralds a new dawn of sustainable and high-yield bioproduction processes, shaping the horizon of biotechnological innovations to come.