D-Cellobiose-[13C12]

D-Cellobiose-[13C12], a labeled disaccharide with diverse scientific applications, particularly in biochemical and metabolic research, offers a multitude of uses. Here are the key applications:

Metabolic Flux Analysis: Delving into the intricate world of metabolic flux analysis, D-Cellobiose-[13C12] acts as a crucial component in tracing carbohydrate metabolism and unraveling the intricate flow of carbon through metabolic pathways. By integrating this labeled sugar into biological systems, researchers can meticulously track its assimilation and conversion into other metabolites, offering profound insights into the dynamic nature of cellular metabolism and the profound impact of genetic or environmental alterations.

Stable Isotope Labeling: In the realms of proteomics and metabolomics, D-Cellobiose-[13C12] plays a pivotal role in stable isotope labeling, enabling the precise quantification and analysis of biomolecules. The incorporation of the 13C isotope results in distinct shifts in mass spectrometry, facilitating accurate differentiation between labeled and unlabeled compounds. This technique not only permits precise measurements of metabolic rates but also aids in identifying crucial metabolic intermediates, painting a comprehensive picture of molecular processes.

Gut Microbiome Research: Acting as a valuable tool in the exploration of the gut microbiome and its involvement in carbohydrate digestion, D-Cellobiose-[13C12] opens a window into intricate biological processes. By introducing this labeled sugar to experimental models, researchers can closely monitor its breakdown by gut bacteria and subsequent absorption, shedding light on the contributions of specific microbial communities to carbohydrate metabolism and their profound implications for host health.

Glycoscience Studies: In the realm of glycoscience, researchers harness the power of D-Cellobiose-[13C12] to delve into the enzymatic processing and utilization of disaccharides. This labeled sugar serves as a cornerstone in exploring enzyme kinetics, substrate specificity, and the synthesis of oligosaccharides, unraveling the complex world of carbohydrate chemistry. Such investigations play a pivotal role in advancing our understanding of biological mechanisms and hold promise for diverse applications in biotechnology and medicine, driving innovation in the field.