D-glucose-[2,5,6-13C3]

D-glucose-[2,5,6-13C3] is a stable isotope-labeled glucose molecule with extensive applications in scientific research. Here are its key applications:

Metabolic Flux Analysis: D-glucose-[2,5,6-13C3] is pivotal in unraveling metabolic pathways by tracing how cells metabolize glucose. The incorporation of labeled carbon atoms into metabolic intermediates allows researchers to map out intricate metabolic networks. This technique sheds light on cellular energy production, bioenergetics, and metabolic disorders, offering invaluable insights into how cells handle glucose and revealing potential targets for therapeutic intervention.

Nuclear Magnetic Resonance (NMR) Spectroscopy: In NMR spectroscopy, D-glucose-[2,5,6-13C3] serves as a potent signal source, enabling detailed study of glucose dynamics and interactions within various biological systems. The isotopic labeling enhances signal resolution, allowing scientists to dissect overlapping signals and achieve precise structural elucidation. This capability is crucial for exploring the roles of carbohydrates in biological processes and understanding complex biomolecular interactions.

Positron Emission Tomography (PET) Imaging: For PET imaging, D-glucose-[2,5,6-13C3] acts as a valuable tracer for monitoring glucose uptake and metabolism in living tissues. This is particularly relevant in cancer research, where altered glucose metabolism is a hallmark of tumor cells. The labeled glucose enables real-time visualization and quantification of metabolic changes, providing critical insights into tumor behavior and aiding in the development of innovative diagnostic and therapeutic strategies.

Isotope Dilution Mass Spectrometry (IDMS): In IDMS, D-glucose-[2,5,6-13C3] is employed as an internal standard to accurately quantify glucose levels in biological samples. Its use helps mitigate variations in sample preparation and enhance the reliability of analytical measurements. This application is crucial for clinical diagnostics and metabolic research, ensuring precise and consistent glucose quantification, which supports accurate disease diagnosis and monitoring of metabolic changes.