D-fructose-[3-13C] 1,6-bisphosphate sodium salt

D-fructose-[3-13C] 1,6-bisphosphate sodium salt, a labeled biochemical reagent, finds diverse applications in research. Here are the key applications:

Metabolic Flux Analysis: Used in metabolic flux analysis, this compound facilitates the investigation of carbon flow through glycolytic pathways. By tracing labeled carbon atoms, researchers can monitor the dynamics of intermediate metabolites and their conversions. This intricate process aids in unraveling the intricacies of cellular metabolism and pinpointing bottlenecks within metabolic pathways.

NMR Spectroscopy: Employed in nuclear magnetic resonance (NMR) spectroscopy, D-fructose-[3-13C] 1,6-bisphosphate sodium salt plays a crucial role in exploring enzyme mechanisms and interactions. The 13C label generates distinct NMR signals, simplifying the tracking and analysis of biochemical changes. This detailed approach enables comprehensive structural and functional characterization of metabolic enzymes, shedding light on their intricate functions.

Stable Isotope Labeling: Acting as a stable isotope-labeled standard, this compound supports comparative studies in systems biology. Researchers leverage it to label specific metabolic pathways and accurately quantify metabolomics data. Its application is vital for validating metabolic models and unraveling pathway dynamics under varying conditions, contributing to a deeper understanding of complex biological systems.

Enzyme Kinetics: In the realm of enzyme kinetics, D-fructose-[3-13C] 1,6-bisphosphate sodium salt proves invaluable for studying the dynamics of glycolysis-related enzymes. By providing a labeled substrate, scientists can precisely monitor reaction rates and enzyme-substrate interactions. This intricate data is essential for deciphering enzyme regulation mechanisms and designing targeted inhibitors or activators for therapeutic interventions.