4-nitrophenyl α-D-mannopyranoside-[1,2-13C2]

4-Nitrophenyl α-D-mannopyranoside-[12-13C2], a specialized chemical substrate utilized in biochemical and biophysical investigations, boasts a myriad of applications. Here are the key applications:

Enzyme Kinetics: Embarking on the intricate journey of enzyme kinetics, this compound stands as a fundamental tool for deciphering the nuanced behaviors of α-mannosidase enzymes. By incorporating the [12-13C2]-labeled variant, researchers unveil the potentials of NMR spectroscopy to meticulously monitor enzymatic activity with unparalleled precision. This methodology yields comprehensive insights into enzyme mechanisms and substrate interactions, illuminating the inner workings of these biological catalysts with unmatched depth and clarity.

Structural Biology: Positioned at the vanguard of structural biology, 4-nitrophenyl α-D-mannopyranoside-[12-13C2] takes a prominent role in crystallographic explorations of enzyme-substrate complexes. The isotopically labeled substrate plays a pivotal role in unraveling active sites and binding interactions through the fusion of X-ray diffraction and NMR methodologies. This symbiotic approach proves instrumental in crafting potent enzyme inhibitors and optimizing catalytic efficiency, propelling advancements in the domain of structural biology with resounding impact and significance.

Metabolic Flux Analysis: Within the realm of metabolic flux analysis, the labeled substrate emerges as a pivotal instrument for scrutinizing carbohydrate metabolism within cellular systems. By tracing the journey of labeled carbon atoms, scientists can map the intricate flow of metabolites along specific pathways, unveiling the dynamic intricacies of metabolic responses. This approach not only enriches our comprehension of metabolic dynamics but also streamlines metabolic engineering strategies, ushering in fresh perspectives on cellular metabolism with remarkable insight and innovation.

Diagnostic Assays: In the realm of diagnostic assays, 4-nitrophenyl α-D-mannopyranoside-[12-13C2] assumes a pivotal role in detecting and quantifying α-mannosidase activity in biological specimens. This application holds particular significance in the diagnosis of lysosomal storage disorders, where enzyme dysfunction culminates in the accumulation of substrates. The precision and sensitivity facilitated by the labeled substrate elevate the reliability of these assays, underscoring its pivotal role in the realm of diagnostic medicine with unparalleled acumen and efficacy.