N-acetyl-D-neuraminic acid-[2,3-13C2]

N-acetyl-D-neuraminic acid-[2,3-13C2] is a versatile isotopically labeled derivative of sialic acid, commonly applied in various biochemical and medical research endeavors. Here are the key applications of N-acetyl-D-neuraminic acid-[2,3-13C2]:

Metabolic Labeling Studies: Employed extensively in metabolic labeling studies, N-acetyl-D-neuraminic acid-[2,3-13C2] plays a pivotal role in investigating the metabolism and integration of sialic acid into glycoproteins and glycolipids. By introducing this labeled component into cellular systems, researchers can meticulously trace the metabolic pathways and fluxes utilizing advanced mass spectrometry techniques. This methodology aids in unraveling the intricate dynamics of sialic acid metabolism and its multifaceted functions within cellular systems.

Structural Biology: In the realm of structural biology, N-acetyl-D-neuraminic acid-[2,3-13C2] serves as a potent tool for examining the structure and interactions of sialic acid-incorporating biomolecules. The isotopic labeling with 13C facilitates the application of NMR spectroscopy for obtaining comprehensive structural insights. This application proves invaluable for delving into the conformational alterations and binding interactions occurring within sialic acid-associated proteins and receptors, shedding light on their intricate structural features.

Infectious Disease Research: Vital for infectious disease research, N-acetyl-D-neuraminic acid-[2,3-13C2] is utilized to explore the involvement of sialic acid in pathogen-host interplays. Numerous viruses, bacteria, and toxins exploit sialic acid residues on host cells for adherence and cellular entry. By leveraging the labeled derivative, researchers can dissect these intricate interactions, potentially paving the way for developing inhibitors to disrupt pathogenic entry mechanisms.

Drug Development: A cornerstone in drug development efforts, the isotopically labeled N-acetyl-D-neuraminic acid-[2,3-13C2] plays a crucial role in designing sialic acid-based therapeutics. Researchers leverage this compound for screening potential drug candidates targeting sialic acid-processing enzymes or sialic acid-binding receptors. This strategic approach aids in the crafting of novel antiviral, antibacterial, and antineoplastic agents that exploit the intricate sialic acid pathway, offering new avenues for therapeutic interventions.